Mouse Thioredoxin Reductase (TrxR) ELISA Kit

Cat.#:

JL13379

Regulatory Status:

RUO

Alternative:

cytoplasmic; Gene associated with retinoic and IFN-induced mortality 12 protein; Gene associated with retinoic and interferon-induced mortality 12 protein; Gene associated with retinoid IFN induced mortality 12 protein; GRIM 12; GRIM-12; GRIM12; KDRF; KM

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.075ng/mL

Specificity:

This kit recognizes Mouse TrxR in samples. No significant cross-reactivity or interference between Mouse TrxR and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| TrxR

Thioredoxin reductases (TR, TrxR)are enzymes that reduce thioredoxin (Trx).Two classes of thioredoxin reductase have been identified: one class in bacteria and some eukaryotes and one in animals. In bacteria TrxR also catalyzes the reduction of glutaredoxin like proteins known as NrdH.Both classes are flavoproteins which function as homodimers. Each monomer contains a FAD prosthetic group, a NADPH binding domain, and an active site containing a redox-active disulfide bond.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.44	1.87	1.29	0.83	0.58	0.38	0.27	0.08
Corrected OD	2.36	1.79	1.21	0.75	0.5	0.3	0.19	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse TrxR spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-104	101
Cell Culture Supernates(n=8)	96-108	104

| Linearity

Samples were spiked with high concentrations of Mouse TrxR and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	87-96	90-110
1 : 2	Average (%)	91	92	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	108